Archives

Biophysical and In Vitro Comparability Analysis of an AAV Vector Produced by the Baculovirus/SF9 System and HEK Triple Transfection System

Eric D. Horowitz. Presented at the 2018 Annual Congress of the European Society of Gene and Cell Therapy.

Insect Media Evaluation for Cell Growth and rAAV Production in an SF9-Baculovirus System

Taylor Polhemus, Hui Wu, David Dismuke, Christopher J. Morrison. Presented at the 2018 Annual Congress of the European Society of Gene and Cell Therapy. 

Engineering of 5′ UTR to Control the Expression and Incorporation Level of VP1 During rAAV Vector Production Using a Baculovirus System

Sylvain Cecchini, Jeffrey Slack, Luis Maranga. Presented at the 2018 Annual Congress of the European Society of Gene and Cell Therapy.

Empty Virions In AAV8 Vector Preparations Reduce Transduction Efficiency And May Cause Total Viral Particle Dose-Limiting Side-Effects.

Gao K, Li M, Zhong L, Su Q, Li J, Li S, He R, Zhang Y, Hendricks G, Wang J, Gao G. Mol Ther Methods Clin Dev. 2014;1(9):20139.

Production and discovery of novel recombinant adeno-associated viral vectors.

Mueller C, Ratner D, Zhong L, Esteves-Sena M, Gao G. Curr Protoc Microbiol. 2012 Aug;Chapter 14:Unit14D.1.

Reproducible high yields of recombinant adeno-associated virus produced using invertebrate cells in 0.02- to 200-liter cultures.

Cecchini S, Virag T, Kotin RM. Hum Gene Ther. 2011 Aug;22(8):1021-30.

Large-scale recombinant adeno-associated virus production.

Kotin RM. Hum Mol Genet. 2011 Apr 15;20(R1):R2-6.

A simplified baculovirus-AAV expression vector system coupled with one-step affinity purification yields high-titer rAAV stocks from insect cells.

Smith RH, Levy JR, Kotin RM. Mol Ther. 2009 Nov;17(11):1888-96.

Producing recombinant adeno-associated virus in foster cells: overcoming production limitations using a baculovirus-insect cell expression strategy.

Virag T, Cecchini S, Kotin RM. Hum Gene Ther. 2009 Aug;20(8):807-17.

Strategies for manufacturing recombinant adeno-associated virus vectors for gene therapy applications exploiting baculovirus technology.

Negrete A, Kotin RM. Brief Funct Genomic Proteomic. 2008 Jul;7(4):303-11.


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